Journal of Food, Agriculture and Environment




Vol 4, Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Correlation of PCR and IFAS with eggplant bioassays for identification of Clavibacter michiganensis subsp. sepedonicus


Author(s):

Ahmed L. Abdel-Mawgood 1, 3*, Thomas L. German 2

Recieved Date: 2006-01-08, Accepted Date: 2006-03-19

Abstract:

Correlation was examined among immunoflurorescence, eggplant pathogenicity bioassays and a PCR assay for identification of Clavibacter michiganensis subsp. sepedonicus (Cms) using the novel primer pair Cms A/Cms B designed based upon the sequence of the 16S rRNA gene. These primers identified 11 Cms isolates in eggplant extracts and an additional 14 isolates in pure culture, and results of PCR using these primers correlated 100% with results of IFAS and eggplant pathogenicity assays. CmmClavibacter michiganensis subsp. michiganensis, did not produce symptoms in eggplant and was not identified by IFAS or PCR. These results demonstrate that PCR with the primer pair Cms A/Cms B can be as a complimentary test to IFAS and eggplant pathogenicity assays for the detection of Cms isolates found in the Midwestern United States.

Keywords:

Bacterial ring rot, seed potato certification, PCR, IFAS, eggplant bioassay, Cms, Corynebacterium sepedonicum, Clavibacter, Cmm, potato pathogen, 16s rRNA gene


Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Agriculture
Pages: 179-182


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