Journal of Food, Agriculture and Environment




Vol 4, Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Application of molecular markers for hybrid maize (Zea mays L.) identification


Author(s):

A. L. Abdel-Mawgood 1, 3*, M. M. M. Ahmed 2, B. A. Ali 2

Recieved Date: 2006-01-14, Accepted Date: 2006-03-18

Abstract:

Three inbred lines of maize (L1, L2 and L3) and two F1 hybrids derived from them (H1 and H2) are used as a model system for examining the capability of molecular markers for the identification of these hybrids. Several molecular techniques, RAPD, SSR and 18s rRNA gene, were used in this study. Two primers out of five RAPD markers used in this study showed polymorphism. One of the polymorphic primers (Primer 1) was able to identify H2 hybrid from the two inbred lines involved in this hybrid. This primer showed the pattern of inheritance as expected from Mendelian inheritance. However, the same primer was not able to identify the H1, because there was one extra band in one parent that was not inherited in this hybrid. Primer 5 was able to identify the two hybrids from the pattern of bands in the inbred lines involved. The polymorphic SSR primers used in this study showed unclear pattern of inheritance of the number of repeats. Concerning the 18s rDNA no polymorphism between the inbred lines was noticed even after restriction digestion of the amplified fragment.

Keywords:

RAPD, SSR, 18s rDNA gene, maize, inbred lines, hybrids


Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Agriculture
Pages: 176-178


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :