Journal of Food, Agriculture and Environment

Isolation and screening of cellulolytic fungi from soil and evaluation of its potential to utilize different cellulosic material for the production of ethanol

Author(s):

Dhanpal B. Chavan, Purthviraj S. Sadar

Recieved Date: 2022-09-02, Accepted Date: 2022-12-22

Abstract:

Cellulose is an abundant biopolymer on our earth; it is made of glucose polymer. Cellulose is not readily utilized by microorganisms, many fungi and bacteria utilize cellulose and obtain energy from it. Certain fungi like Aspergillus niger, Rhizopus sp. and Fusarium sp. are capable to produce cellulase and releasing fermentable sugar. In this study, Fusarium sp. DBC1 was isolated and its enzyme activity was checked using cellulose as a substrate. Enzyme activity was 1.29 ± 0.005 U/ml. This fungus generates fermentable sugar. When grown on pure cellulose, it generates 60 mg/ml of reducing sugar whereas on filter paper it generates 40 mg/ml and on algae cellulose it releases about 20 mg/ml. The sccharified pure cellulose, filter paper and algae cellulose when, subjected to fermentation by Saccharomyces cerevisiae up to one week. After incubation, ethanol was qualitatively checked using idoform test. The distillation of fermented medium was carried out and a pure distillate obtained. The percentage of ethanol in distillate was checked using a specific gravity method. Pure cellulose recorded 14% alcohol, whereas filter paper recorded 9% and algae cellulose only 3 % ethanol.

Keywords:

Ethanol, saccharification, fermentation, cellulosic, Fusarium sp

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Journal: Journal of Food, Agriculture and Environment
Year: 2023
Volume: 21
Issue: 1
Category: Environment
Pages: 77-80

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