Journal of Food, Agriculture and Environment
Vol 12,
Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Assessments of biodiversity of ornamental Ficus species based on EST markers and morphological traits
Author(s):
Naglaa Mohamed Esmaiel 1,
Kamal Fouad Abdellateif 2, Samah Mohamed Mahmoud Eldemery 3, Adel Moustafa Zakri 4, Abdullah Abdulaziz Al-Doss 4, Mohamed Najeb Barakat 4, 5*Recieved Date: 2014-02-20, Accepted Date: 2014-04-18
Abstract:This study was conducted to characterize and detect genetic relatedness among 21 ornamental species of the genus Ficus grown in Alexandria, Egypt, using the powerful combination of morphological and EST molecular markers. Results of the two-way hierarchical cluster analysis of the 21 Ficus species and the morphological traits indicated that the twelve morphological traits were separated into two main clusters in the first way of hierarchical clustering, while, in the other way of the two-way hierarchical cluster analysis, the Ficus species were distributed according to this analysis into five clusters. The relationships among the 21 Ficus species were estimated by a UPGMA cluster analysis of genetic similarity matrices. Cluster analysis using EST data grouped the 21 Ficus species into four main clusters with simple matching (SM) similarity coefficient ranging from 0.56 to 0.99. The highest similarity was found between F. altissima and F. laurifolia (0.99), which have also very high value of similarity according to the morphological traits analysis, and the lowest similarity was obtained between F. altissima and F. afzelii (0.56). The polymorphic information content (PIC) per primer pairs ranged from 0.78 to 0.89 with an average of 0.84. PIC values were positively correlated (r = 0.61) with number of amplified alleles per primer.
Keywords:EST markers,
Ficus species, genetic diversity, morphological traits, two-way hierarchical cluster analysisJournal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Environment
Pages: 932-938
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