Journal of Food, Agriculture and Environment

Breeding for micronutrient improvements in rice (Oryza sativa L.) for better human health

Author(s):

Muhammad Ashfaq ¹*, Muhammad Saleem Haider ¹, Abdus Salam Khan ², Muhammad Ali ¹, Amna Ali ¹, Urooj Mubashar ³

Recieved Date: 2014-02-13, Accepted Date: 2014-04-24

Abstract:

A good number of genotypes (local + exotic) were used for the improvement of micronutrient values in Basmati rice germplasm through conventional breeding programme for better human health. Analysis of variance and correlation of different morphological traits of these genotypes were studied. All the genotypes showed significant variations. On the other hand, positive significant associations were also observed, i.e. seed length showed positive association with seed thickness, length width ratio and 1000 grain weight (r = 0.2644**, r = 0.4072**, r = 0.3691**). Seed width showed positive association with seed thickness, 1000 grain weight and seed thickness showed positive association with 1000 grain weight (r = 0.5059**, r = 0.3131**, r = 0.2929**). The variety KBNT lpa1-1 having high micronutrient value was used for the screening of other genetic material and for the production of new rice lines through recombination with high number of micronutrient value and good yield potential. For this purpose, the screening was done through PCR analysis by using different number of SSR primers through CTAB method. The overall objective of the study was to screen out the diverse germplasm lines with high micronutrients value for further production of new desirable rice lines through conventional breeding programme. A good number of new rice lines (IR-64, Basmati-385 and Basmati-370) were screened out by studying the various phenotypic and genotypic traits for the later generations study and selection to increase the homozygosity of each rice line. The mean number of alleles per locus was 3.66, and the average number of polymorphism information content (PIC) values (0.561) was also observed that showed genetic variation among all genotypes. It is concluded that all the genotypes showed almost significant genetic variability on the basis of their seed morphological traits.

Keywords:

Oryza sativa, germplasm, SSR primer, micronutrients, desirable traits

---

Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Agriculture
Pages: 365-369

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :