Journal of Food, Agriculture and Environment




Vol 12, Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Breeding for micronutrient improvements in rice (Oryza sativa L.) for better human health


Author(s):

Muhammad Ashfaq 1*, Muhammad Saleem Haider 1, Abdus Salam Khan 2, Muhammad Ali 1, Amna Ali 1, Urooj Mubashar 3

Recieved Date: 2014-02-13, Accepted Date: 2014-04-24

Abstract:

A good number of genotypes (local + exotic) were used for the improvement of micronutrient values in Basmati rice germplasm through conventional breeding programme for better human health. Analysis of variance and correlation of different morphological traits of these genotypes were studied. All the genotypes showed significant variations. On the other hand, positive significant associations were also observed, i.e. seed length showed positive association with seed thickness, length width ratio and 1000 grain weight (r = 0.2644**, r = 0.4072**, r = 0.3691**). Seed width showed positive association with seed thickness, 1000 grain weight and seed thickness showed positive association with 1000 grain weight (r = 0.5059**, r = 0.3131**, r = 0.2929**). The variety KBNT lpa1-1 having high micronutrient value was used for the screening of other genetic material and for the production of new rice lines through recombination with high number of micronutrient value and good yield potential. For this purpose, the screening was done through PCR analysis by using different number of SSR primers through CTAB method. The overall objective of the study was to screen out the diverse germplasm lines with high micronutrients value for further production of new desirable rice lines through conventional breeding programme. A good number of new rice lines (IR-64, Basmati-385 and Basmati-370) were screened out by studying the various phenotypic and genotypic traits for the later generations study and selection to increase the homozygosity of each rice line. The mean number of alleles per locus was 3.66, and the average number of polymorphism information content (PIC) values (0.561) was also observed that showed genetic variation among all genotypes. It is concluded that all the genotypes showed almost significant genetic variability on the basis of their seed morphological traits.

Keywords:

Oryza sativa, germplasm, SSR primer, micronutrients, desirable traits


Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Agriculture
Pages: 365-369


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