Journal of Food, Agriculture and Environment




Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Heat exposure upregulates expression of cytokines and aggravates lung injury in Klebsiella pneumoniae – Challenged rats


Author(s):

Jiansheng Li 1, 2*, Xue Mei 1, Hongyan Zhou 1, Yanxia Zhang 1, Suyun Li 2

Recieved Date: 2013-06-14, Accepted Date: 2013-10-29

Abstract:

To demonstrate impact of thermal environment on klebsiellar pneumonia and the underlying molecular mechanism, Wistar rats were randomly divided into four groups: 1) no-treatment control (n = 12), 2) heat-treatment alone (n = 12), 3) K. pneumoniae pneumonia alone (n = 12), and 4) K. pneumoniae pneumonia plus heat treatment (n = 12). White blood cells (WBC) and polymorphonuclear neutrophils (PMN) percentage in peripheral blood were examined. Pathological changes of lung tissue were observed. IL-6, TNF-αand ICAM-1 in the bronchoalveolar lavage fluid were evaluated by radioimmunoassay. IL-1β, TNF-αand IL-6 protein expressions in lung tissue were analyzed by immunohistochemistry staining. The expression of IL-1βmRNA was measured by RT-PCR. Histopathological study indicated that there was pronounced damage in the heat + pneumonia group. Significant elevation of TNF-α, IL-6 and levels in the bronchoalveolar lavage fluid and lung tissue was observed in the heat + pneumonia group compared to the heat-alone group or the pneumonia-alone group. ICAM-1 level in the bronchoalveolar lavage fluid was higher in the heat+ pneumonia group compared to the heat-alone group. However, there was no difference in IL-1βlevel between the heat + pneumonia group and the pneumonia-alone group. The main findings of our study were that heat exposure increased levels of inflammation cytokines and aggravated pathological injury of lung tissue in Klebsiella pneumoniae pneumonia rats.

Keywords:

Klebsiella pneumoniae pneumonia, interleukin -1β, Interleukin-6, tumor necrosis factor-α, intercellular adhesion molecule-1, lung injury


Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 139-144


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