Journal of Food, Agriculture and Environment

New micro bioassay sandwich to detection allelopathic activity from Ipomoea batatas (L.) Lam.


Maykel Hernández-Aro 1, Ricardo Hernández-Pérez 2 *, Dagoberto Guillén-Sánchez 3

Recieved Date: 2015-06-16, Accepted Date: 2015-09-17


The inhibition (or stimulation) of seed germination was the aspect most widely used by bioassays to determine the allelopathic activity of plants. In this work, extracts of Ipomoea batatas (L.) Lam. to different concentrations were evaluated against weed species Amaranthus palmeri S., using the new sandwich micro bioassay modified, for detecting the allelopathic effect from this donor. Through this assay a delay in germination index (GI) of 68.4% was observed when the maximum concentration was applied as well as a decrease in germination rate (GSI) (9.5-1.5) seeds per day, with increasing concentration of the extracts (1-4% w/v), respectively. Response was calculated as inhibitory index (RI) (0.65-0.76) for the proportion of germinated seeds (GI) and a delay in germination rate (0.85 to 0.90). The use of this method allowed the optimization of resources per analysis, using a minimum quantity of donor extract and a greater number of analyses per unit time. However, its effectiveness must be validated with a greater number of seeds and types of species donor.


Micro bioassay, sandwich, allelopathy, weed, Amaranthus palmeri

Journal: Journal of Food, Agriculture and Environment
Year: 2015
Volume: 13
Issue: 3&4
Category: Agriculture
Pages: 45-48

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