Journal of Food, Agriculture and Environment

Genotyping of five Chinese local pig breeds focused on meat quality by using PCR-RFLP based on halothane and Mx1

Author(s):

Ze-Meng Feng, Xiaoli Zhou, Hua Shao, Xiang-Feng Kong *, Yu-Long Yin, Ruilin Huang*

Recieved Date: 2012-07-10, Accepted Date: 2012-10-01

Abstract:

In recent years, due to the increasing demand for pork with high quality, a more convenient and fast way to test pigs is required for breeding, which enhances investigations on genetic variations associated with meat quality. So far, many genetic variations associated with meat quality have been investigated, such as halothane (Hal) and Myxovirus resistance protein 1 (Mx1). The current studies focus on the use of RCR-RFLP of Hal and Mx1 for evaluating meat quality traits of several Chinese local pig breeds (Bama mini-pig, Huanjiang mini-pig, Lantang pig, Ningxiang pig and Xiangxi black pig including Daheping black pig, Pushi black pig and Taoyuan black pig) and Landrace pig (a foreign pig breed). The results indicated that the genotypes of Chinese local pig breeds were mainly shown as Hal^NN, while rarely in Hal^Nn, rather than Halⁿⁿ. The recessive allele (n) frequency in Chinese local pig breeds was significantly lower than that in Landrace pig. The recessive allele (B) frequency in the sixth intron of Mx1 in Chinese local pig breeds was generally higher than that in Landrace pig. The B frequency in the ninth intron of Mx1 was commonly higher in both Chinese local pig breeds and Landrace pig. In general, these findings have manifested that Chinese local pig breeds, particularly Xiangxi black pig and Ningxiang pig, have a better meat quality. 

Keywords:

PCR-RFLP, meat quality, Chinese local pig, halothane, Mx1

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 840-845

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