Journal of Food, Agriculture and Environment

Gene expression profiles in porcine intestinal epithelial cells treated with arginine using a microarray technique

Author(s):

Liming Zeng ^{1, 2, 3}, Bie Tan ², Yulong Yin ², Xiangfeng Kong ², Zemeng Fen ², Jun Fang ¹*, Xiangyang Lu¹*

Recieved Date: 2012-06-20, Accepted Date: 2012-10-03

Abstract:

L-arginine has been demonstrated to stimulate cell proliferation and prevent LPS-induced death of intestinal cell. To identify genes responsed to arginine treatment in intestinal cell, gene expression profiles were analyzed using high-density oligonucleotide microarray in porcine intestinal epithelial cells (IPEC-1) treated with 100 (control) or 350 µM arginine. Results showed that expression levels of 306 genes were up-regulated and 280 genes down-regulated in IPEC-1 cultured in medium containing 350 µM arginine compared with that of the control. Although the biochemical functions of some genes had not previously been defined, we were able to identify some up-regulated genes that are known to play important roles in transportion (e.g. ATP6V1G2, SLC9A4), metabolism (e.g. AKR1C4, GHRL), transcription and signal transduction (e.g. PTGFR, OCT2), signaling and proliferation (e.g. UPTI, FGFR1), cell cycle and apoptosis (e.g. DRAM1, GADD45A), cytoskeletal and cell adhesion (e.g. DNMBP, ITGB2) and immune response (e.g. OAS1, IL15), and some down-regulated genes that are related to transportion and metabolism (e.g. SLC25A25, COX1), cytoskeletal and cell adhesion (e.g. PROC, COL10A1) and immune response (e.g. SLA-DMA, CD80). The RT-PCR analysis demonstrated that 350 µM arginine treatment increased ITGB (109%), AKRIC4 (367%), ATP6VIG2 (196%), DRAMI (88%), GHRL (243%) and SLC9A2 (138%), which were almost consistent with the data obtained from the microarray analysis. Our findings provided a molecular mechanism to explain cytoprotective effects of arginine and laid the foundation for future research on cellular mechanisms of arginine regulating cell growth and proliferation. 

Keywords:

Arginine, IPEC-1, nutrition, gene expression profile, pig, microarray

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 834-839

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