Journal of Food, Agriculture and Environment
Vol 8,
Issue 3&4,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Intestinal and renal Type II NaPi co-transporter gene expression patterns in growing pigs fed with different levels of dietary calcium
Author(s):
Lingyuan Yang 1, 2,
Shun-xiang Wang 1, Gong Min 3, Xu-gang Shu 4, Zhi-qiang Liu 1, Tiejun Li 1, Yulong Yin 1*Recieved Date: 2010-07-14, Accepted Date: 2010-10-30
Abstract:In this experiment, 25 barrows pigs (20.9 ± 0.95 kg) were fed with diet containing 5 different levels of Ca (0.29, 0.44, 0.60, 0.75 and 0.91%) through different supplementations of limestone powder. Each trial lasted for 35 days long and the pigs were slaughtered on the last day. Jejunum and kidney samples were collected. The amounts of transcription of NaPi-IIc and NaPi-IIa in the samples were measured using fluorescent quantitative PCR assay. According to the NRC, the requirement of Ca for the pigs (20-50 kg) is about 0.6%, so the group of pigs fed with 0.60% Ca level was selected as the calibrator. Compared with Ca level of 0.60%, pigs fed diets with Ca level of 0.44%, 0.75% and 0.91% enhanced mRNA transcription of NaPi- IIc in jejunum (1.49, 13.60 and 7.65) and NaPi-IIa in kidney (2.15, 6.53 and 8.48). Pigs fed with Ca level of 0.29% decreased production of mRNA transcription of NaPi-IIa.
Keywords:Calcium,
phosphorus, growing pigs, sodium-dependent Pi absorption, gene expressionJournal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Agriculture
Pages: 882-886
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