Journal of Food, Agriculture and Environment

Evaluation of physicochemical characteristics of microemulsion formulation of rotenone and its insecticidal efficacy against Plutella xylostella L.(Lepidoptera: Plutellidae)

Author(s):

Siti Nurulhidayah Ahmad ¹, Md. Touhidul Islam ¹, Dzulkefly Kuang Abdullah ², Dzolkhifli Omar ¹*

Recieved Date: 2012-07-10, Accepted Date: 2012-09-20

Abstract:

Five microemulsion formulations, M₂, M₄, M₇, M₉ and M₁₃ of rotenone, were used to evaluate the physicochemical characteristics and its insecticidal efficacy against early third instar larva of diamondback moth (DBM) compared with commercial rotenone- Saphyr^® 6.6 EC. Five series of concentrations, 54.6, 109.2, 163.8, 218.4 and 273 ppm, were used in this experiment. Mortality of nymph was recorded at 96 h after dipping treatments were performed. All formulations tested showed excellent stability by centrifugation, following by 3 months at room temperature without phase separation. They also showed lower surface tension compared with water. The particle size of the formulation indicates that all formulations are in range of microemulsion (5- 100 nm) except formulation M₄. The lowest miscibility of rotenone was obtained from formulation M₃ that could dissolve only 31.53% rotenone compared with other formulations. Formulation M₉ showed the highest mortality with 100% achievement and the lowest LC₅₀ value (87.22 ppm) of diamondback moth (DBM). This novel approach for insecticide formulation of rotenone might be useful for the control of DBM. This study will provide the maximum utilization of botanical insecticide concerning environmental friendly strategy.

Keywords:

Microemulsion, rotenone, Plutella xylostella, particle size, surface tension, LC₅₀

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 384-388

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