Journal of Food, Agriculture and Environment

Effect of dietary L-glutamine supplementation on edema disease induced by Escherichia coli

Author(s):

Xiaosong Wu ^1*#, Wenkai Ren ^2#, Shu Xu-gang ³, Ruan Zheng ³, Sisi Cai ⁴, Jinping Deng ¹, Dingding Su ⁵

Recieved Date: 2011-12-12, Accepted Date: 2012-04-26

Abstract:

This study was conducted to test the hypothesis that the dietary glutamine supplementation had various effects on oedema disease induced by Escherichia coli (E. coli). Total of 162 KunMing mice were randomly assigned to glutamine group 1 (1.0% glutamine+basal diet, n = 45), glutamine group 2 (1.0% glutamine+basal diet, n = 45), control group 1 (1.22 % alanine+basal diet, n = 36) and control group 2 (1.22% alanine+basal diet, n = 36). After 3 days of adaptive feeding and a 7-days treatment period with the experiment feed, all mice were challenged by intraperitioneal injection of E. coli O₁₃₉ at LD50 (2.53×10⁸ CFU/ml). The mortality after twenty hours of injection was calculated from glutamine group 2 and control group 2. Serum concentrations of platelet-activating factor (PAF), interleukin (IL)-2, interleukin (IL)-10, secretory immunoglobulin A (sIgA), superoxide dismutase (SOD) activity, cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) were measured in glutamine group 1 and control group 1 in a ten-hours interval for three times.  Serum concentrations of PAF in 1.0% glutamine group were significantly lower (P<0.01) than that in 1.22% alanine group at ten and thirty hours after initial injection and lower (P<0.05) than the control group twenty hours after first injection. Additionally, the supplementation of glutamine increased the SOD activity significantly (P<0.05) in the first twenty hours after initial injection. However, the SOD activity in the glutamine group was lower (P<0.05) than in the control group later. Furthermore serum concentration of cAMP had no difference at the first ten hours after injection, whereas the cAMP concentrations in the glutamine group was significantly lower (P<0.01) at twenty hours after initial injection and lower (P<0.05) than in the control group thirty hours after first injection. Meanwhile, glutamine supplementation had little effect on the mortality of mice, serum IL-2, sIgA and cGMP. In conclusion, dietary glutamine supplementation could partially attenuate the damage caused by oedema disease, but had little effect on the clinical result. 

Keywords:

Glutamine, oedema disease, Escherichia coli, platelet-activating factor

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Agriculture
Pages: 726-731

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