Journal of Food, Agriculture and Environment

The effect of chemical inducers and UV radiation on morphogenesis ability of Brassica napus L. in somatic tissue culture

Author(s):

Natalija Burbulis, Aušra Blinstrubienė

Recieved Date: 2011-01-02, Accepted Date: 2011-04-15

Abstract:

In vitro culture techniques offer a new breeding strategy that may lead to new desirable cultivars. Besides, in vitro techniques also have an enormous potential of broadening the scope of mutation breeding by accelerating the process and thus leading to more economical outputs. The effect of chemical inducers (abscisic acid, isonicotinic acid, oxalic acid, salicylic acid) and ultraviolet irradiation on cultured somatic tissues of Brassica napus was investigated. The experiments were carried out with four winter oilseed rape cultivars ‘Libea’, ‘Valesca’, ‘Siska’, ‘Liprima’ and four spring oilseed rape cultivars ‘Liaison’, ‘SW Landmark’, ‘Heros’, ‘Ural’. Explants were cultured on the medium supplemented with different tested acids concentrations or treated by ultraviolet light immediately after isolation. Generally tested oilseed rape cultivars were able to form organogenic structures in the presence of chemical inducers in induction media. However, higher concentrations of exogenous acids significantly decreased (especially isonicotinic and salicylic acids) or completely inhibited morphogenesis process of tested oilseed rape cultivars. The shoots regeneration frequency decreased with increasing duration of UV radiation and LD₅₀ was estimated to correspond to an exposure of 45 min. The procedures for adventitious shoot regeneration described in this paper could potentially be useful for producing large populations of regenerants for in vitro mutation-selection, which increases the probability of obtaining mutants with desired traits such as disease resistance.

Keywords:

Brassica napus L., chemical inducers, cultivar, in vitro, regenerants, UV radiation

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 263-267

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