Journal of Food, Agriculture and Environment

Plant regeneration in inflorescence culture of chrysanthemum (Dendranthema x grandiflora (Ramat.) Kitamura)

Author(s):

Danuta Kulpa

Recieved Date: 2010-09-17, Accepted Date: 2011-01-20

Abstract:

The aim of this study was to determine the effects of initial explants, growth regulators and light on the organogenesis in the cultures of chrysanthemum inflorescence parts. Ray flowers, disc florets and flower bottom of Erica variety of chrysanthemum were used for initiation of culture. Explants placed on the MS medium supplemented with auxins (1.0 mg·dm^-3 IAA, IBA, NAA and 2.4-D) or with a combination of auxin NAA (from 1.0 to 5.0 mg·dm^-3) and cytokinine BAP (from 0.1 to 2.0 mg·dm^-3). Half of the cultures were carried out in the light of the intensity 50 PAR while the other half in the dark. The obtained results showed that ‘Erica’ can be successfully regenerated in vitro culture from inflorescence parts. Disc florets were found to have the poorest regeneration ability under experimental conditions and the greatest ability was observed in the case of receptacle. The highest number of regenerated plants was obtained, irrespective of the used initial explants on MS medium with BAP (2 mg·dm^-3) and an auxin NAA (5 mg·dm^-3). Light stimulated plant regeneration in all experimental combinations.

Keywords:

Dandranthema, inflorescence culture, growth regulators, light

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Environment
Pages: 715-718

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