Journal of Food, Agriculture and Environment

Vol 5, Issue 3&4,2007
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Applications of fluorescent in situ hybridization (FISH) in environmental microbiology


João V. B. Souza 1*, Roberto Moreira da Silva Júnior 1, Daniela Koshikene 3, Érica S. Silva 2

Recieved Date: 2007-04-02, Accepted Date: 2007-08-15


Environmental microbiology utilizes indirect methods as microscopy and culture media to its studies. However, these conventional methodologies present problems to quantify and qualify microorganisms and their relation with the environment. The aim of this manuscript was to describe the application of fluorescent in situ hybridization (FISH) in environmental microbiology studies. It was discussed the limitations of conventional studies, the principles of the technique, advances produced by the introduction of this technique in environmental microbiology and also FISH limitations. FISH is a technique that employs fluorescent microscopy to observe specific microorganisms in natural samples identifying them by their nucleotide sequence of ribosomal ribonucleic acid (RNAr). Fluorescent probes, like an intelligent stain, hybridize exclusively with the RNAr of the chosen microorganisms allowing to: I) identify the microorganisms in environmental samples without the utilization of culture media; II) quantify the microorganisms directly in the sample; III) determine the morphology; IV) describe the spatial distribution and V) determine the natural relation between species. The RNAr allows the phylogeny studies due to its conserved and varied regions. FISH methodology has two main parts, the cell preparation and hybridization. The technique is not more complex than the pathology one. In environmental microbiology, FISH works have been carried out with samples originated from sea water, rivers, lakes, biofilms, soil, plants and animals. This molecular tool has allowed the first trusty “in situ” studies in this area.


FISH, environmental microbiology, molecular tools, probes

Journal: Journal of Food, Agriculture and Environment
Year: 2007
Volume: 5
Issue: 3&4
Category: Environment
Pages: 408-411

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :