Journal of Food, Agriculture and Environment

Vol 5, Issue 2,2007
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Biochemical and physiological responses in Wistar rat after administration of puffer fish (Lagocephalus lagocephalus) flesh


Mongi Saoudi 1, Faouzi Ben Rabeh 2, Kamel Jammoussi 3, Abdelwaheb Abdelmouleh 2, Lassaad Belbahri 4*, Abdelfettah El Feki 1

Recieved Date: 2007-01-05, Accepted Date: 2007-03-20


The aim of this study was to evaluate the toxicity of the puffer fish L. lagocephalus after administration to Wistar rats. For this purpose, male wistar rats were fed during two periods of treatment, 48 h and 2 months, with a standard rat diet supplemented with the L. lagocephalus flesh. Various diet compositions were tested: 100% standard rat diet (T); 10% of raw flesh of L. lagocephalus (Lcr); 10% of cooked flesh of L. lagocephalus with or without water of cooking (Lcu+b and Lcu-b respectively). The values were validated by concerning flesh of raw and cooked mule Liza aurata (Mcr and Mcu+b respectively). The evaluation of hematologic parameters showed that Lcr and Lcu+b foods induced in the rats haemolytic anaemia (for the two treatment periods) as well as macrocytic anaemia accompanied with swelling and interruption of erythrocytes maturation. For Lcu+b rats, serum analysis showed disturbance of transaminase activity (aspartate aminotransferase AST and alanine aminotransferase ALT) which could indicate an uncompensated hepatic cellular lesions. Indeed, a significant reduction of serum alkaline phosphatase (ALP) rate was noted after 2 months of treatment. These results were confirmed by a hyperproduction of thiobarbituric acid reactive substances (TBARS) in the liver tissue suggesting hepatic oxidative damages. However, the serum cholinesterase activity (AChE) showed no significant variation between rat groups, suggesting that brain was not altered. These findings were not similar to those of Lcu-b and Lcr. Hence, the cytotoxic factor of L. lagocephalus flesh seems to be water-soluble and heat-stable. In raw flesh, the extraction is less to cause only hematologic effects, whereas, in cooked flesh with the use of water of cooking, the extraction is more important and causes more effects. These results suggested that in diet containing the cooked flesh with water of cooking, the toxic effect is more pronounced than in raw flesh.


Biochemical parameters, hematotoxicity, hepatotoxicity, L. lagocephalus, puffer fish

Journal: Journal of Food, Agriculture and Environment
Year: 2007
Volume: 5
Issue: 2
Category: Food and Health
Pages: 107-111

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :