Journal of Food, Agriculture and Environment

Vol 4, Issue 3&4,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Production of a recombinant vp1 protein of the chicken anaemia virus for the development of an enzyme-linked immunosorbent assay


I. O. M. Soliman 1*, M. A. M. Lila 1, M. Z. Saad 1, R. A. Rahim 2

Recieved Date: 2006-05-27, Accepted Date: 2006-08-12


In this report, we describe the development of ELISA assay for CAV in Malaysia using recombinant VP1 capsid protein of the CAV that reduce the vast cost and reliable method for diagnosis. The VP1 gene was cloned and expressed in prokaryotic expression vector pRSET–B. The VP1 protein expression in E. coli was achieved as a recombinant protein containing immunogenic epitope. The protein expressed was detected by anti- VP1 monoclonal antibody with an apparent molecular weight of 50 kDa. Batch fermentation was used to scale up production of the protein in E. coli, the final VP1 protein yield produced was 64 µg/ml. The protein expressed has been tested as an antigen for detection of antibody to CAV in infected chicken. An enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of serum antibody to CAV and compares its performance with that of the commercial ELISA using serum samples from commercial and SPF flocks. When serum samples from infected chicken were tested, the sensitivity of the assay was found to be 93.3% compared to 100% for commercial ELISA. On the other hand, the specifity of the indirect ELISA (100%) had a greater value over the specifity of commercial ELISA (95%). Furthermore, the ELISA test requires serum samples to be diluted at 1:100 compared to 1:50 for commercial ELISA.


Chicken anaemia virus, VP1, purification, batch fermentation, indirect ELISA

Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 3&4
Category: Food and Health
Pages: 50-55

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :