Journal of Food, Agriculture and Environment
Vol 4,
Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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In vitro zygotic embryo germination of Calamus latifolius Roxb. and Calamus tenuis Roxb.
Author(s):
Bharati Meitram,
G. J. Sharma *Recieved Date: 2005-11-27, Accepted Date: 2006-03-18
Abstract:A highly economical medium for in vitro zygotic embryo germination of two priority species of rattan (Calamus latifolius Roxb. and Calamus tenuis Roxb.) has been used for the first time. Seedling growth of embryo explants in three different media, namely, Murashige and Skoog medium (MS), woody plant medium (WPM) and Y3 medium supplemented with plant growth regulators (PGRs) was investigated. Embryo explants inoculated onto WPM supplemented with various concentrations of PGRs produced increased seedling growth compared to the other two media used. Benzylaminopurine (BAP), 4.54-8.88 µM, produced higher shoot length when treated singly than in combination with 0.54-5.57 µM α-naphthalene acid (NAA). Root length was found to be highest in 0.54 µM NAA supplemented WPM in both the species. Combination with BAP produced no significant increase in root length. Multiple shoot induction occurred best on WPM supplemented with a combination of 23.25 µM kinetin (Kn) and 2.26 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Complete plantlet development occurred faster in WPM in both the species.
Keywords:In vitro propagation,
priority species, Calamus latifolius Roxb., Calamus tenuis Roxb.Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Environment
Pages: 306-309
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