Journal of Food, Agriculture and Environment




Vol 4, Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Cryopreservation of potato (Solanum tuberosum L.) shoot tips using vitrification and droplet method


Author(s):

Artur Kryszczuk 1*, Joachim Keller 2, Marion Grübe 2, Ewa Zimnoch-Guzowska 1

Recieved Date: 2005-12-11, Accepted Date: 2006-03-17

Abstract:

The experiments were done to compare two methods of potato (Solanum tuberosum L.) shoot tips cryopreservation: the vitrification and the droplet method. Maximum regrowth rates of all evaluated varieties (from 42 to 88%) were obtained using the vitrification method. The droplet method was also successful after cold hardening of in vitro plants (regrowth rates from 5 to 45%). A comparison of two regeneration media: liquid and solid and two methods of cryoprotectants sterilization, namely autoclaving vs. filter sterilization were also done. In dependence on the cryopreservation method and modification of some steps of the procedure, statistically important differences of shoot tips survival and regeneration among varieties were observed. Results indicate the higher efficiency of vitrification. On the other hand, the droplet method is much simpler. It can be expected that some modifications of the method combining various steps of the originally published protocols to simplify the procedure, but still keeping high regeneration rates are possible.

Keywords:

Cryopreservation, potato, droplet method, vitrification, gene bank, long-term maintenance


Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Agriculture
Pages: 196-200


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :