Journal of Food, Agriculture and Environment

Analogous effect of preheating on chilling sensitivity of mung bean seedlings and membrane viscosity to effects of ABA and uniconazole treatments

Author(s):

Kamonwan Chucheep ¹, Sirichai Kanlayanarat ², Tomoaki Matsuo ^{1, 3*}

Recieved Date: 2005-03-18, Accepted Date: 2005-09-03

Abstract:

Many plants of tropical and subtropical origin are susceptible to chilling injury after exposure to nonfreezing temperatures below 12°C. Proper treatments with preheating, ABA and uniconazole have frequently been known to alter the chilling sensitivity of several fruits and vegetables during cold storage. Investigation of a factor(s) which increases during preheating and causes a modification in the chilling sensitivity of mung bean (Vigna radiata L.) seedlings was carried out in this study.
   Keeping mung bean seedlings at 4°C for more than 3 days prevented successive hypocotyl growth and caused hypocotyl wilting and browning at the site of hypocotyls. Three hours and 6 h of preheating the seedlings at 40°C effectively improved the chilling damage compared with the control seedlings (4°C for 5 d in the dark). Application of preheating at 40°C for 6 h reduced the rate of ion leakage from the seedlings segments remarkably, in contrast to untreated ones. Both the chemicals, ABA and uniconazole, also suppressed ion leakage from the segments remarkably. Maintenance of high cell viability in the preheated seedlings was observed compared with the control seedlings. Membrane viscosity measured with the fluorescence spectrophotometer, using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a probe for monitoring the relative fluidity of the isolated cytoplasmic membrane, showed that at the lower temperatures, preheating and two plant growth regulators, ABA and uniconazole, induced changes in membrane fluidity, as compared with the control. This modification of membrane fluidity is suggested to be related to a protein stabilizer(s). There was no difference observed among protein profiles in SDS-PAGE of the proteins extracted from membrane fractions. Therefore, the interaction of heat shock-induced proteins with cytoplasmic membranes may be very sensitive under conditions of SDS-PAGE.

Keywords:

Chilling injury, heat shock treatment, mung bean seedling, ion leakage, cell viability, membrane viscosity, fluorescence probe, abscisic acid, uniconazole

---

Journal: Journal of Food, Agriculture and Environment
Year: 2005
Volume: 3
Issue: 3&4
Category: Agriculture
Pages: 110-116

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :