Journal of Food, Agriculture and Environment

A simple protein extraction method for proteomic studies on olive leaves

Author(s):

Nasir S. A. Malik ^*, Joe M. Bradford

Recieved Date: 2005-01-12, Accepted Date: 2005-03-10

Abstract:

Researchers have encountered difficulties in extracting proteins from olive leaves because of the high content of interfering compounds in this tissue. Recently, after testing several methods, a phenol-SDS method was devised to extract proteins from olive leaves for proteomic studies. This method is tricky and the use of phenol, a very corrosive agent, requires careful handling. We therefore tested the phenol-SDS method along with a slightly modified standard method that gave equal, or better, results on two dimensional electrophoresis gels. This simpler and more convenient method of protein extraction from olive leaves is described here.

Keywords:

Protein extraction, olive leaves, proteomics, 2-D electrophoresis

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Journal: Journal of Food, Agriculture and Environment
Year: 2005
Volume: 3
Issue: 2
Category: Agriculture
Pages: 246-248

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