Journal of Food, Agriculture and Environment

Evaluation of DNA integrity for PCR-based detection of transgenic maize along the production chain of animal feed

Author(s):

Gabriela M. Garrido-Ortiz, Noelia N. Barriga-Medina, Darío X. Ramírez-Villacís, Germán Romo, Antonio Leon-Reyes

Recieved Date: 2020-02-02, Accepted Date: 2020-03-22

Abstract:

Several countries have included regulations for planting and trading genetic modified organisms (GMOs). These regulations require the industry to declare the presence of transgenic components on food labels. Therefore, it is important to standardize short and efficient methods for the detection of GMOs in food processing and final products. Maize is one of the largest transgenic crops in the world, and, since its contribution of carbohydrates as source of energy, it is mainly employed in animal feed. The objective of this study was to assess DNA vulnerability by heat and pressure in three feed manufacturing processes: expansion, pelleting and extrusion. Samples analyzed in this study reached a maximum temperature of 120°C and 35 bars of pressure. DNA was isolated from each production stage of the three feeds which were produced using transgenic maize. DNA extraction based on cetyltrimethylammonium bromide (CTAB) was employed since it is a simple and inexpensive method. Then, by using Polymerase Chain Reaction (PCR) of CaMV 35S promoter region was used to detect the presence of transgenic material, and maize Invertase gene as amplification control. Results showed that on each production stage of the three types of feed, genomic maize DNA can be isolated from feed samples with enough quality for the PCR amplification. In this study, we concluded that CTAB method and the amplification of the CaMV 35S promoter gene can be used for traceability of transgenic maize during production of animal feed.

Keywords:

Maize, animal feed, transgenic, CTAB method, Invertase gene, Cauliflower Mosaic Virus promoter gene

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Journal: Journal of Food, Agriculture and Environment
Year: 2020
Volume: 18
Issue: 2
Category: Agriculture
Pages: 73-78

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