Journal of Food, Agriculture and Environment

Evaluation of the glyphosate (N-phosphonomethyl glycine) biodegradation potential by autochthonous soil fungi from southern Brazil

Author(s):

Fernanda N. Demichelli ¹,  Juciana C. Cazarolli ², Diego dos Santos ¹, Thiago B.  Bitencourt ¹, Gilmar Franzener ¹, Gabriela S. Moura ¹, Catia T. Passos ¹,  Luisa H. Cazarolli ¹*

Recieved Date: 2018-07-17, Accepted Date: 2018-09-20

Abstract:

Glyphosate is one of the most commonly used pesticides in Brazil, and it is related to health issues and environmental problems. Thus, the present study aimed to isolate and identify fungi from  glyphosate contaminated soil as well as investigate their glyphosate degradation potential. The soil samples were collected from a farm in Laranjeiras do Sul, PR, Brazil and transferred to a flask with mineral medium (MM) supplemented 100 mg.L^-1 of glyphosate. Then, we performed three subsequent transfers (1 mL) to the same media, every five days, at 28°C. After that, aliquots were transferred to Sabouraud agar supplemented with glyphosate (100, 200, 300 mg.L^-1) and incubated for five days at 28°C. After incubation, fungal colonies were counted and, reisolated according to morphology in Sabouraud agar supplemented with 300 mg.L^-1 of glyphosate. For the radial mycelial growth, the fungi were incubated in MM agar with 300 mg.L^-1 of glyphosate as the single carbon source for 14 days, at 28°C. The radial mycelial growth was daily measured and the growth rate was calculated by linear regression. Eventually, eight out of the 14 fungi isolated had the capacity to grow in high glyphosate concentrations. It was possible to identify at the genus level 10 microorganisms and Aspergillus sp. showed the highest growth rate in high glyphosate concentrations, demonstrating that this microorganism could be used in further bioremediation studies.

Keywords:

Glyphosate, fungi, pesticides, bioremediation, microorganisms, herbicide

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Journal: Journal of Food, Agriculture and Environment
Year: 2018
Volume: 16
Issue: 3&4
Category: Environment
Pages: 65-68

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