Journal of Food, Agriculture and Environment

Novel enzymatic extraction and purification method for cyclic adenosine monophosphate (cAMP) from Ziziphus jujuba

Author(s):

Chunxia Wang ^{1, 3, 4}, Xueqin Gao ⁴, Haikuan Wang ^{1, 3, 4}, Yihan Liu ^{1, 2, 4}, Lianxiang Du ⁴, Hongbin Wang ^{1, 3, 4}, Fuping Lu ^1,2,4

Recieved Date: 2013-06-29, Accepted Date: 2013-10-20

Abstract:

Cyclic adenosine monophosphate (cAMP) is an important component in the regulation of intracellular metabolism and biological function. The cAMP content of jujube (Ziziphus jujuba Mill, Chinese date) is particularly abundant and stable, more than any of the other plants researched so far. In this paper we report the development of a novel enzymatic extraction method for cAMP from Ziziphus jujuba fruit (jujube) paste. The paste was hydrolyzed at 50°C with 2.5 g/L pectinases (pectin lyase, cellulose, and hemicellulose, optimal ratio 4:2:1) for 3 h. The yield efficiency of jujube juice achieved 70%, and most importantly, the extracted cAMP content increased from 108 to 157 mg/kg. After resin adsorption, elution with formic acid, and filtration through a 150 Da nanofiltration membrane, the crude cAMP content was increased from 30% to 36% with the growing rate of 20% compared with the single pectinase treatment. The purification percentage of cAMP using silica gel column chromatography was as much as 97%.

Keywords:

Jujube, plant extract, cyclic adenosine monophosphate, silica gel column chromatography, nanofiltration, purification

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 469-474

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