Journal of Food, Agriculture and Environment

Identification of bovine, buffalo and yak milk with polymerase chain reaction – restriction fragment length polymorphism

Author(s):

Yan B. Jiang ¹, Hai Wang ¹, Dong J. Hou ¹, Yan Yin ¹, Tong Li ¹, Xiao L. Tian ², Xin N. Li ², Lei Yu ¹*

Recieved Date: 2013-06-20, Accepted Date: 2013-10-30

Abstract:

Buffalo and yak milk which are more expensive than bovine milk have been accepted by parts of people in China for their high nutrition, while the milk adulteration puzzled us a lot. With close genetic relationship, bovine milk, buffalo milk and yak milk are not so easy to be detected, nor can we find a fitful method through current journals. In this study, polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) was applied to identify bovine, buffalo and yak milk. With universal primers targeting mitochondrial 12S rDNA, 375 bp fragments were obtained with bovine and yak DNA, and a 376 bp fragment was obtained with buffalo DNA through PCR. Digestion with restriction endonucleases Msap1 I and Pac I were applied. When digested with Msap1 I, bovine PCR product was digested into 90 bp and 285bp fragments while buffalo PCR productyielded 91 bpa0141bp and 144 bp fragments. Thus, Msap1 I was used to detect the bovine milk in buffalo milk. For identification of yak milk and bovine milk, double digestion with Msap1 I and Pac I were applied and the yak PCR product yielded 82bp, 90bp and 203bp fragments, while the bovine PCR product still yielded 90 bp and 285bp fragments. It was shown that 285 bp, 144bp and 203 bp DNA fragments were the characteristic fragments of bovine, buffalo and yak, respectively. This method can detect adulterate buffalo milk and yak milk mixed with bovine milk with a detection limit lower than five percent.

Keywords:

Milk identification, bovine, buffalo, yak, PCR-RFLP

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 455-457

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