Journal of Food, Agriculture and Environment

Purification and characterisation of laccase from Lactarius volemus and its application in removal of phenolic compounds from fruit juice

Author(s):

Hayrunnisa Nadaroglu ¹*, Esen Tasgin²

Recieved Date: 2013-05-19, Accepted Date: 2013-10-22

Abstract:

Laccases are multicopper oxidases (EC 1.10.3.2.), widely distributed in fungi, higher plants and bacteria. In this research, a laccase from Lactarius volemus (Russulaceae) was purified by 73.08 fold using precipitate of saturation (NH₄)₂SO₄, DEAE-cellulose and Sephacryl S200. It was shown thatpurified enzyme was homogeneous in term of SDS-PAGE with molecular mass estimated of 30 kDa. The pH was optimal at 4.0, enzyme was stable at pH 3.0-5.0 and optimal temperature was at 50^°C. Cu²⁺ and Fe²⁺ were activated to the purified laccase enzyme, and it was partially inhibited by 5 mM concentration of some metal salts and EDTA, urea, SDS, L-cysteine, dithioeritritol and β-mercaptoethanol. In addition, it was investigated whether the purified and characterized laccase enzyme would remove the phenolic compound of some fruit juices which are responsible for haze formation and browning during storage. Their application led to reduction of total phenolic compounds for apple, apricot, cherry and grape juice. As a result, the effect of these enzymatic treatments on total phenol content and clarity of the juice were evaluated using a central composite design. This laccase enzyme had potential application in food processing.

Keywords:

Lactarius volemus (Russulaceae), laccase, purification, characterization, fruit juice

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 109-114

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