Journal of Food, Agriculture and Environment

Extraction of agarwood (Aquilaria crassna) oil by using supercritical carbon dioxide extraction and enzyme pretreatment on hydrodistillation 

Author(s):

Nuttawan Yoswathana

Recieved Date: 2013-01-02, Accepted Date: 2013-04-28

Abstract:

Agarwood (Aquilaria crassna) is a fragrant wood containing economically important essential oil. It has been traded for use as incense, perfume and traditional medicine. In this study, the effect of three different methods for extraction of agarwood oil and the chemical compositions of extracted agarwood oil were studied. Conventional hydrodistillation method had two major disadvantages namely; long extraction time of about 5 days and high energy consumption with low yield (0.075% w/w). Pretreatment of agarwood with technical enzymes before hydrodistillation affected the extractability of essential oil positively (0.20% w/w). Extraction of agarwood oil using supercritical carbon dioxide at 600 bar resulted higher yield (0.47% w/w) compared to hydrodistilation at distinct shorter extraction time of 3 h and process temperature (85°C). Analyzing of the extracts using Gas Chromatograph/Mass Spectrometer (GC/MS) had shown that the composition of essential oils differed from each other. The major compounds in essential oil from hydrodistillation were selina-3,7(11)-diene (17.21%), δ-selinene (12.36%), 1,3,5-trimethyl-6-methyldiene-tricyclo [3.2.1.0(2,7) [oct6-3-en-8exo-ol (10.26%) and 4a,5-Dimethyl- 3-(1-methyldiene)-4,4a,5,6,7,8-hexa hydro-2(3H)-napthalene (9.6%). Pretreatment of agarwood with technical enzymes resulted aristol-9-en-8-one (28.77%), β-guaiene (14.94), guaia-3,9-diene (14.32%) and 7,7-dichlorobicycl0 [3.2.0] hept-2-en- 6-one (7.58%) as the principal substances.  For supercritical carbon dioxide (ScCO₂) extraction (600 bar, 85°C, 3h) of agarwood was three substances; furoscrobiculin B (27.98%), furosardorin A (19.07%), and bicyclo[4.4.0]des-5-ene, 1,5-dimethyl-3-hydroxy-8-(1-methylene-2-hydroxyethyl-1) (18.68%) as the main substances in agarwood oil. 

Keywords:

Agarwood oil, supercritical carbon dioxide (ScCO₂), hydrodistillation

---

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Environment
Pages: 1055-1059

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :