Journal of Food, Agriculture and Environment




Vol 12, Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone residues


Author(s):

Jin-qing Jiang

Recieved Date: 2014-01-20, Accepted Date: 2014-03-08

Abstract:

A broad specificity ELISA based on monoclonal antibody for multiresidue determination of fluoroquinolone (FQ) residues has been developed. For this purpose, pefloxacin (PEF) was employed to synthesize the immunogen, and cell fusion technology was used to produce anti-PEF monoclonal antibody. Five hybridoma cell lines named PEF-1, PEF-2, PEF-3, PEF-4 and PEF-5 were screen out, their corresponding mAbs were of the IgG1 isotype with k light chain, and the Kaffs of all mAbs were between 2.2 and 4.5×109 L/mol. Based on the square matrix titration, an icELISA method was established, and its dynamic range for pefloxacin in assay buffer was from 0.008 to 96 ng/mL, with LOD and IC50 value of 0.005 ng/mL and 0.6 ng/mL, respectively. The results of this assay showed a high cross-reactivity to ciprofloxacin (80%), enrofloxacin (70%), difloxacin (68%), sarafloxacin (63%), and norfloxacin (59%). Under 20-fold dilution in authentic milk sample, the matrix effects actually can be ignored. The results suggest this immunoassay can be used for simultaneous detecting six kinds of FQs in milk samples.

Keywords:

Pefloxacin, fluoroquinolones, monoclonal antibody, indirect competitive ELISA, milk


Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Food and Health
Pages: 70-73


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :