Journal of Food, Agriculture and Environment




Vol 12, Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Antioxidant capacity of Elaeagnus angustifolia L. and investigation of eosin y as the fluorescent probe in ORAC method


Author(s):

Gorkem Yalcin *, Ozlem Sogut

Recieved Date: 2014-01-02, Accepted Date: 2014-03-16

Abstract:

Elaeagnus angustifolia L. (oleaster) is one of the widely consumed medicinal plant in Turkey. It is used in the treatment of some diseases in folk medicine. Leaf, seed, peel, and mesocarp parts of oleaster were extracted with methanol (100%, v/v) in ultrasonic bath in the present study, and antioxidant capacities of these parts’ extracts of Elaeagnus angustifolia were evaluated by using oxygen radical absorbance capacity assay (ORAC). The leaf part of Elaeagnus angustifolia L. (428 µmol Trolox Equivalent (TE) g-1) possessed stronger antioxidant properties in comparison with other parts. Fluorescein is the most used fluorescent probe in ORAC method. In this assay, eosin y, which is a derivative of fluorescein, was compared with fluorescein as the fluorescent probe. The detection limit (DL) and quantification limit (QL) were 3.65 and 11.06, 7.12 and 21.57 µM TE for ORAC-fluorescein (ORAC-FL) and ORAC-eosin y (ORAC-EY), respectively.

Keywords:

ORAC, eosin y, Elaeagnus angustifolia L., oleaster


Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Food and Health
Pages: 51-54


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