Journal of Food, Agriculture and Environment

Genotyping of Salmonella enterica collected from poultry farms located in Cairo, Egypt by Multiplex-PCR

Author(s):

Ihab Mohamed Moussa ¹, Ashgan Mohamed Hessain ², Mustafa Abudo Gassem ³, Abdullah Abdulaziz Al-Arfaj ¹, Ismail Al Hazmi Mohamed ³

Recieved Date: 2013-11-20, Accepted Date: 2014-01-12

Abstract:

The objective of the present study is to detect the incidences of Salmonella enterica from different poultry farms located in Egypt on molecular basis. During the summer of 2012, a total of 1800 cloacal swabs were collected from poultry farms located in Cairo, Egypt to be subjected for isolation of Salmonella enterica. Moreover, a total of 300 samples of poultry and poultry products were collected from different retail establishment markets in Cairo, Egypt including, 150 local whole frozen chickens, 50 imported whole frozen chickens, 100 local chicken cut samples. The highest rate of isolation (8%) was obtained from imported frozen chickens and local chicken cuts, followed by local frozen chickens (6.66%) and finally rectal swabs from apparently healthy chickens (6.4%). Salmonella Typhimurium and Salmonella Enteritidis were most frequent among the total Salmonella isolates. Multiplex-PCR for the rapid detection of Salmonella Typhimurium and Salmonella Enteritidis from field samples especially after preenrichment on Rappaport-Vassiliadis (RV) selective broth (PCR-RV), revealed the same positive samples. Therefore, PCR-RV technique is rapid, time saving and applicable to detect Salmonella serovars directly from chicken samples. Moreover, detecting Salmonella Typhimurium and Salmonella Enteritidis by this assay was carried out within 2 days opposed to 5-6 days by the bacteriological and serological methods.

Keywords:

Salmonella enterica, Salmonella Typhimurium, Salmonella Enteritidis enrichment, Multiplex-PCR

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 1
Category: Agriculture
Pages: 195-198

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