Journal of Food, Agriculture and Environment

Evaluation of 8-hydroxylquinoline toxicity on different developmental stages of loach (Misgurnus anguillicaudatus) using acute toxicity test, hepatase activity and comet assay

Author(s):

Ping Nan, Shuaiguo Yan, Jianjun Chen, Xiaohua Xia, Qiyan Du, Zhongjie Chang*

Recieved Date: 2012-09-10, Accepted Date: 2013-01-20

Abstract:

This study was a preliminary step to evaluate the toxicity effect of 8-hydroxylquinoline (8-HOQ) on different developmental stages of loach (Misgurnus anguillicaudatus) through using the methods of acute toxicity test, hepatase activity and comet assay. The results of acute toxicity test indicated that 8-HOQ had obvious toxicity effect on loach at its different developmental stages, and larva loach was more sensitive to 8-HOQ-toxicity than adult fish. Biochemical assays in adult loach showed that 8-HOQ also induced change in the activities of the glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) of fish hepatopancreas. With the increase of the treatment-concentration and treatment-time of 8-HOQ, the GPT and GOT activity of loach liver were decreased obviously, there was signicant difference between control group and each treatment group (p<0.05). The results of enzyme activity assays in adult loach suggest that 8-HOQ exposure may induce physiology toxicity in hepatocyte (liver damage) of tested fish. Subsequent comet assay test in adult loach showed that 8-HOQ could induce DNA damage of fish liver cells. With the increase of the treatment-concentration and treatment -time of 8-HOQ, three comet parameters (trailing rate, tail length, tail moment) of liver cells were increased significantly and showed a concentration-effect and time-effect. There was signicant difference (p<0.05) between control group and each treatment group in three comet parameters. The results of comet assay test showed that 8-HQ exposure may induce genetic toxicity (mutagenic and genotoxic effect) on the hepar cells in adult loach. In conclusion, those results of above all suggest that 8-HOQ may become toxic chemical contaminant in environment and threaten health of aquatic and other organisms. 

Keywords:

Loach (Misgurnus anguillicaudatus), 8-hydroxylquinoline, GOT, GPT, comet assay

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Environment
Pages: 771-776

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