Journal of Food, Agriculture and Environment

Vol 11, Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young infloresceances in orchardgrass (Dactylis glomerata L.)


Ibrahim Atis 1*, Ersin Can 1, Nafiz Celiktas 1, Ruştu Hatipoglu 2

Recieved Date: 2012-09-06, Accepted Date: 2013-01-08


This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l-1 of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that  there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment  varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1- 115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg-1 of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish  and 1.6 shoots  per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on  MS medium containing 4 mg 1-1 of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies. 


Orchardgrass, MS medium, 2,4,5-T., tissue culture, plant regeneration

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Agriculture
Pages: 564-567

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :