Journal of Food, Agriculture and Environment




Vol 11, Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Effects of production factors on the antioxidant activity of protein hydrolysates from little hairtail (Trichiurus haumela) of East China Sea


Author(s):

Ting Jin *, Yuxue Wu

Recieved Date: 2012-10-10, Accepted Date: 2013-01-29

Abstract:

This study examined the effects of production factors such as hydrolysis time, enzyme-to-substrate ratio, temperature and pH, on the antioxidant activity of hydrolysates from little hairtail. The ABTS radical scavenging activity was as a target to evaluate the antioxidant activity of hydrolysate. The protein hydrolysates were freeze-dried and characterized with respect to molecular mass distribution and amino acid compositions. The results showed that the hydrolysate with high antioxidant activity was produced successfully by hydrolyzing protein of little hairtail with Alcalase. The ABTS radical scavenging activity was up to 76.5% under optimum conditions, which were as follows: the protein was hydrolyzed by Alcalase for 3 h, enzyme to substrate ratio 0.6%, temperature 55°C and pH 7.5. The molecular mass of freeze-dried hydrolysates was between 446 and 7635 Da, which indicated that the protein of little hairtail was hydrolyzed by Alcalase to low molecular peptides which had antioxidant activity. The amino acid compositions of little hairtail hydrolysates included antioxidative-related animo acids, hydrophobic amino acids and others. These amino acids constituted peptides which lead to hydrolysate with high antioxidant activity. The protein hydrolysate of little hairtail (Trichiurus haumela) with high antioxidant activity could be used as antioxidant ingredient in various food products. 

Keywords:

Little hairtail (Trichiurus haumela), antioxidant activity, production factors, Alcalase


Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Food and Health
Pages: 95-98


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :