Journal of Food, Agriculture and Environment




Isolation, identification and analysis of DDT-degrading bacteria for agriculture area improvements


Author(s):

Pannapa Powthong 1*, Bajaree Jantrapanukorn 1, Pattra Suntornthiticharoen 2

Recieved Date: 2015-09-10, Accepted Date: 2015-12-23

Abstract:

Dichlorodiphenyltrichloroethane (DDT) is an organochlorine pesticide that can persist in the environment resulting in environmental problem with chronic effects on human and animal health. Biodegradation is a potential method to detoxify the recalcitrant compounds. In this study, the potential for biodegradation of DDT through enrichment and isolation of DDT-degrading bacteria from soil and water in agricultural area was done. Microorganisms grew in minimal media with DDT as the only carbon source. Six from 40 bacterial isolates were selected by increasing concentrations of DDT. The DDT degradation was quantified by the enrichment cultures degrading S4-67.95%, S13-65.05%, S37-65.45%, S39-67.25%, W30-65.20% and W37-67.55% of the initial amount of DDT, respectively, in 7 days. Further, the optimal of temperature, pH, and salinity of the isolate has also been investigated and revealed that an optimum temperature at 37–45ºC was able to growth at a wide range of NaCl and pH. These promising DDT-degrading isolates were tentatively identified biochemical and MALDI-TOF MS characterization as 3 groups: Pseudomonas stutzeri (3 isolates), Pseudomonas aeruginosa (2 isolates) and Bacillus firmus (1 isolate).

Keywords:

DDT, biodegradation, pesticide, bioremediation, organochlorine compounds


Journal: Journal of Food, Agriculture and Environment
Year: 2016
Volume: 14
Issue: 1
Category: Environment
Pages: 131-136


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :