Journal of Food, Agriculture and Environment

LSU nrDNA of uncultured endophytic fungi amplified from the blossom of Gastrodia elata

Author(s):

Ji-Chuan Kang ¹*, Ting-Chi Wen ¹, Pin Guan ², Jin He ²

Recieved Date: 2012-06-20, Accepted Date: 2012-10-04

Abstract:

This is the first report of the existence of fungal species of Botryotinia, xylariales, Phytophthora, Fusarium and Armillaria on the flower tissue of traditional Chinese medicinal (TCM) plant Gastrodia elata. Random polymerase chain reaction (PCR) amplification of the large-subunit nrDNA sequences of the nuclear ribosomal RNA genes from the “impure”plant genomic DNA isolated from the flower tissue of G. elata resulted in 7 amplified fragments which were subsequently sequenced. Via BLASTn search and phylogenetic analysis of these DNA sequences along with their closest allies in GenBank, they were identified as the members of Botryotinia, xylariales, Phytophthora, Fusarium and Armillaria. The molecular detection and identification of fungal DNA in the host plant provided additional data on the mycota of G. elata. The large-subunit (28S) nrDNA amplification from nature directly and subsequent sequencing and phylogenetic analysis were found to be a fast and reliable technique for the rapid identification of uncultured fungal taxa and was also shown to be an invaluable tool for the discovery of new and/or uncultured taxa. 

Keywords:

Molecular detection, uncultured endophytic fungi, Gastrodia elata, large-subunit(28S) nrDNA, phylogenetic analysis

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Environment
Pages: 1194-1196

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