Journal of Food, Agriculture and Environment

Anatomical structure analysis and classification of in vitro meristem regenerated plum leaf and stem tissues and cells by means of TEM electronmicroscopy and image analysis

Author(s):

Jakab-Ilyefalvi Zsolt ¹ *, Doru Pamfil ¹, Doina Clapa ²

Recieved Date: 2012-07-01, Accepted Date: 2012-10-03

Abstract:

A combination of Murashige-Skoog‘s medium and N⁶ benzylaminopurine  (BAP) was supplied to meristem shoot tips which were excised from heat therapy treated in vitro plum plants (Prunus domestica, L.). After successful  meristem initiation and regeneration process,  in vitro  regenerated  leaf and stem tissues were sampled in order to complete an analytic histological study on leaf and stem tissue characteristics. The objective of our studies was to develop a method for general classification of the in vitro propagated plum leaf and stem cells, the geometrical  characterization of cell shape morphology, showing a specific cell type to which a group of tissue type belongs. A second goal was the evaluation of callose deposition process, sieve element formation at the early stage of plant development, callose deposal being a physiological parameter for stress factor study in plant (wounding, thermal stress, chemical stress). The average cell cross sectional area  was in palisade cells 142.33 µm², epidermis adaxial side  624.14 µm², spongy mesophyll  364.16 µm²  and companion cells 215.85 µm². Leaf cell perimeter average values for the two cultivars were: upper epidermis 593.38 µm², epidermis adaxial side  624.14 µm², spongy mesophyll 364.16 µm² and companion cells 215.85 µm² . While some sieve element plates were occluded in high percent (34%), others showed just minor callose depositions (3.8%). Callose deposition was clearly visible  in both of the studied in vitro regenerated plum plants tissues. Initially, callose was deposited starting from the cell membrane. For most sieve plates, this callose deposition process was sufficient to occlude a great part of the S.E. or at least considerably reduce the pore diameters. 

Keywords:

Biometry, cell morphology, image analysis, cell biology, cytology

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 778-783

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