Journal of Food, Agriculture and Environment
Vol 10,
Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Cloning and sequence analysis of F gene of Newcastle disease virus isolated from Guizhou province, China
Author(s):
Ming Wen 1,
Zhen-Tao Cheng 1, Deng-Xiang Zhang 1, Jin-Long Yang 1, 2, Bi-Jun Zhou 1*Recieved Date: 2012-05-11, Accepted Date: 2012-10-04
Abstract:One pair of specific primers was designed and synthesized according to fusion (F) protein gene sequences of Newcastle disease virus (NDV) from GenBank. The fragments of F gene of 8 NDV strains isolated from Guizhou province were amplified by RT-PCR, then the amplified fragments were cloned into pMD-18T vector and the recombinant plasmids were sequenced. The results showed that the F gene from all of the NDV isolates consisted of 1662 bp, coding for 553 amino acid. Neucleotide and amino acid sequence homologies were found to be 84.0-99.7% and 87.5-99.3% respectively; but the amino acid sequence homologies to other NDV strains such as LaSota, B1 and F48E9 were only 87.2%-97.7%. The deduced amino acid sequence of the cleavage site of F protein of the NDV isolates was 112R-R-Q-K/R-R-F117 (7 strains were virulent) and 112G-R-Q-G- R-L117 (1 strain was avirulent). The NDV systemic evolution tree was depicted by MegAlign software and showed that four strains were gene type VII, two strains were gene type II, and two strains were gene type IX.
Keywords:Newcastle disease,
molecular epidemiological investigation, RT-PCRJournal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 484-486
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