Journal of Food, Agriculture and Environment

Membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu: Changes in activity and isoenzyme profiles during maturation

Author(s):

Galila Hassan Onsa ¹, Nazamid bin Saari ^1*, Azizah Osman ¹, Shamsul Bahri ²

Recieved Date: 2002-12-08, Accepted Date: 2003-04-18

Abstract:

Membrane-bound Polyphenol oxidases (mPPO: E.C.1.10.3.2) and peroxidases (mPOD: E.C.1.11.1.7) were isolated from membrane-fractions of Metroxylon sagu employing temperature-induced phase partitioning technique using Triton X-114. The specific activities in unit activity / mg protein and their isoenzyme profile were studied during maturation. The stages of maturity are the young stage (2-4 years old), pre-mature (5-8 years old) and mature stage (9-12 years old). The highest mPPO specific activity was observed at the top log section of young stage and then decreased by 3.0 and 25-fold at premature and mature stage, respectively. On the other hand, mPOD activity was found to be constant at the early stages of maturity up to the premature stage (4-6 years). mPOD activity then increased by 1.5 and 2.9-fold at premature and matured stage respectively. High mPOD activity at mature stage was associated with the appearance of new isoenzymes as well as intensification of the existing one. A change in the polypeptides during maturation was also observed.

Keywords:

Metroxylon sagu, maturation, membrane-bound polyphenol oxidases and peroxidases, activity, isoenzymes profile

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Journal: Journal of Food, Agriculture and Environment
Year: 2003
Volume: 1
Issue: 2
Category: Food and Health
Pages: 60-65

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