Journal of Food, Agriculture and Environment

Genetic relationships among Turkish hexaploid oat genotypes using microsatellite markers

Author(s):

E. Banu Buyukunal Bal *, Pelin Vural

Recieved Date: 2010-08-05, Accepted Date: 2010-11-03

Abstract:

Microsatellites are one of the most powerful marker systems for assesment of genetic diversity of plant species. The objective of this study was to determine the genetic relationships among Turkish hexaploid oat genotypes (Avena sativa L.) using microsatellite markers. Fifteen oat cultivars were analyzed as follows: nine cultivars of Turkish origins, one wild Turkish species, one Turkish breeding line, and four of USA origins. Among 17 locus specific microsatellite primers (AM), six (35%) generated polymorphic amplification products and were used for cultivar identification and determination of genetic relationships. Homozygous and heterozygous alleles were observed for AM1 and AM7 loci. Polymorphism Information Content (PIC) values were estimated within the range of 0 and 0.88, with an average of 0.66. One to 12 alleles were observed with the studied markers. Allele sizes were used to estimate simple matching (SM) similarities followed by the unweighted pair-group method with arithmetic Average (UPGMA). Based on these data, all genotypes were differentiated from each other, except Seydişehir and Yeşilköy 1779 cultivars. In addition, Ankara 76 was found to be the most dissimilar cultivar to all other sampled genotypes. The USA cultivars were closely allied to Turkish cultivars. The results of this study will be the integral part of future oat breeding programs.

Keywords:

Avena sativa L., microsatellites, UPGMA

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Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Agriculture
Pages: 820-823

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