Journal of Food, Agriculture and Environment
Vol 10,
Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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A novel, sensitive, accurate multiplex loop-mediated isothermal amplification method for detection of Salmonella spp., Shigella spp. and Staphylococcus aureus in food
Author(s):
Kan Jiang 1*,
Qinfeng Lv 2, Donglei Zhang 1, Xiang Gao 3, Yi Zhao 3, Mengxian Li 3, Ling Liu 3, Junhua Wu 3, Xiangyi Lu 3, Cheng Luo 3*Recieved Date: 2012-06-12, Accepted Date: 2012-09-27
Abstract:For fast and efficient reasons, a multiplex loop-mediated isothermal amplification (mLAMP) to detect foodborne pathogens was studied. Three sets of LAMP primers for 3 kinds of pathogens were designed from nuc gene of S. aureus, ipaH gene of Shigella spp. and invA gene of Salmonella spp., respectively. The DNA(s) were specifically amplified in the presence of the templates from three foodborne pathogens in a single tube. The sensitivities of the mLAMP method were shown 1000 times higher in average on the detection for the genes than those of the classical PCR methods. The sequences of mLAMP assay-positive pathogen products were 99% fidelity with the individual pathogen DNA sequence by sequencing. The large number of tests with field samples showed that the specificity of the LAMP assay was high, and no cross-reaction of the primers and the templates occurred in the same tube. The mLAMP method is further improved and simplified in the detection of the three foodborne pathogens with great specificity and sensitivity.
Keywords:Foodborne pathogen,
Salmonella spp., Shigella spp., Staphylococcus aureus, multiplex (m)LAMP, food safetyJournal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Food and Health
Pages: 252-256
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