Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Effect of UV-LED irradiation on microbial growth on solid media as a model for food


Nam Yong Kim, Min-Ji Kwon, Dong Sun Lee, Duck Soon An *

Recieved Date: 2012-06-18, Accepted Date: 2012-09-23


An ultra-violet (UV) light emitting diode (LED) was installed in a food container system, and its effect on suppressing microbial growth was tested. As a solid food model, agar medium plates were inoculated with spoilage-related or pathogenic strains of Escherichia coli, Bacillus cereus, B. subtilis, Pseudomonas fluorescens or Saccharomyces cerevisiae. The plates were irradiated intermittently (four cycles of 1 hour on/1 hour off) by the UV-LED in an automatically controlled system and stored at optimal growth temperatures for one or two days, after which the number of colony forming units on the media was determined. The wavelength (280, 365 and 405 nm) and physical location (2 and 5 cm above the plate) of the UV-LEDs were examined as the independent variables affecting the suppression of microbial growth. A wavelength of 280 nm was significantly effective in deterring the outgrowth of all microbial strains, while 365 and 405 nm were not consistently effective. There was no difference in the microbial growth between the two locations. 


Light emitting diode, wavelength, physical location, colony forming unit

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Food and Health
Pages: 129-131

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