Journal of Food, Agriculture and Environment

Characterization and genetic divergence in Stevia rebaudiana (Bert.) Bertoni clones based in agronomical and morphological characteristics

Author(s):

Edson T. Anami, Juliana P. Poletine *, Maria Celeste Gonçalves-Vidigal, Pedro S. Vidigal Filho, Giselly F. Lacanallo, Marcus V. Kvitschal, Adriana Gonela

Recieved Date: 2010-07-13, Accepted Date: 2010-10-28

Abstract:

The aim of this study was to evaluate stevia clones and characterize genetic divergence in order to provide genitors selection for hybridization in breeding programs. In 2004 and 2005 years, a collection of stevia plants phenotypically superior was carried out and 15 superior clones were selected with high concentration of total edulcorant and considerable relation between rebaudioside A/stevioside. Clones were evaluated in field experiments, in randomized complete blocks scheme with three repetitions. Plant height, production of fresh and dry matter, number of branches per plant, concentration of total edulcorant and relation between rebaudioside A/stevioside were evaluated. Data were submitted to joint variance analysis, and respective means were compared by Scott and Knott test. In the evaluation of association effect among characteristics, Pearson’s correlation was used. Clones’ clustering was done by UPGMA method, using Mahalanobis’ generalized distance as genetic dissimilarity measure. ST75, ST86, ST141 and ST145 clones were characterized as most promising for rebaudioside A/stevioside relation, total edulcorant concentration and dry matter production. Plant height and rebaudioside A/stevioside relation were positively correlated, this association being attributed to the previous selection conducted in germplasm. Positive and significant correlation was verified among fresh matter production, dry matter production and number of branches per plant. Ample genetic variability was observed among 15 stevia clones for all evaluated characteristics, and the ones that most contributed for genetic divergence were rebaudiosideo A/stevioside relation (79%) and concentration of total edulcorant (12%). ST75, ST86, ST141 and ST 145 clones presented high mean genetic divergence in relation to the whole genotypic pool studied. Therefore, hybridizations among these clones may provide generation of segregant populations with high genetic potential for superior individuals’ selection. Combinations between ST75 and ST141 clones could propitiate superior segregants in total edulcorant concentration, rebaudioside A/stevioside and dry matter.

Keywords:

Edulcorant, stevioside, genetic divergence, rebaudioside A

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Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Agriculture
Pages: 463-469

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