Journal of Food, Agriculture and Environment
Proteomics analysis of Rhizoctonia leguminicola, the phytopathogenic fungus that produces slaframine and swainsonine
Author(s):
Haili Li 1, 3,
Rui Gao 2, Yuanying Liu 2, Jianhua Wang 1*, Yanchun Hu 1, Zijun Yang 3, Guodong Yang 1, Rebecca Creamer 4Recieved Date: 2012-01-29, Accepted Date: 2012-05-02
Abstract:Rhizoctonia leguminicola, a fungus that produces swainsonine and causes of the black patch disease of red clover, is one of the most frequently used and extensively studied in disease of red clover and animal poisoning problems. The indolizidine alkaloids slaframine and swainsonine are responsible for locoism, which causes considerable economic losses in animals. In the past decades, extensive work has been done on protection of animals from poisoning and detoxifying of swainsonine, but the problem of locoism is still unsolved. However, protein production in R. leguminicola fungi cells are poorly understood, especially during the period of black patch disease pathogenesis and swainsonine production. In the present study, we used two dimensional gel electrophoresis (2-DE) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) to investigate protein expression in R. leguminicola. More than 390 protein spots were detected by 2-DE, these proteins will give better understanding of the molecular mechanism in black patch disease and swainsonine production. Proteomics analysis of isoelectric focusing (IEF) strip gels of whole protein extracts was done from R. leguminicola and 59 proteins were identified. Those proteins are involved in oxidation-reduction process, response to stimulus, hydrolase activity and oxidoreductase activity. To our knowledge, this is the first proteomic map using 2-DE establish to study the extracellular proteins secreted by R. leguminicola. The whole cell proteins were used for searching for proteins possibly involved in the black patch disease of red clover and producing swainsonine of fungus R. leguminicola.
Keywords:Rhizoctonia leguminicola,
phytopathogenic fungi, slaframine, swainsonine, animal, locoismJournal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Environment
Pages: 956-961
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