Journal of Food, Agriculture and Environment




Vol 8, Issue 3&4,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Ribotype and serotype diversity of Escherichia coli isolates from poultry and human sources


Author(s):

Cynthia Sheffield 1, Cecilia Rosario-Cortés 2, Kenneth Bischoff 3

Recieved Date: 2010-07-16, Accepted Date: 2010-11-08

Abstract:

Escherichia coli isolates from the Universidad Nacional Autónoma de México were analyzed to evaluate the genetic relatedness and serotype. Ribotype was determined using the RiboPrinter® Microbial Characterization System following DNA digestion with the restriction enzyme EcoRI. A total of 41 ribogroups were isolated. These segregated into three clusters, one cluster contained only poultry isolates, the second contained only human isolates and the third contained 55% poultry isolates and 45% human isolates. Results showed a high level of ribotypic relatedness within the E. coli isolates that could infect both poultry and human sources. E. coli isolates collected from exclusively poultry or human sources showed a high level of ribotypic diversity. Serotype was determined using antisera against somatic (O) antigens. A total of 22 serotypes were identified, of which six were typical of the avian pathogenic E. coli (APEC) strains and six contained both human and poultry isolates.

Keywords:

Escherichia coli, ribotype, serotype, human, poultry


Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Food and Health
Pages: 211-216


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