Journal of Food, Agriculture and Environment




Vol 8, Issue 3&4,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Determining antioxidant capacity of extracts of Filipendula vulgaris Moench from south-western Romania


Author(s):

Ilinca M. Imbrea *, Monica Butnariu, Alma L. Nicolin, Florin Imbrea

Recieved Date: 2010-07-20, Accepted Date: 2010-10-28

Abstract:

The area taken in the study is located in the south-west of Romania, in the Aninei Mountains. The research we undertook this year led to a screening of the pharmaco-dynamic combinations with real data on the antioxidant capacity as well as on the metal content. The results of the study confirmed that metals interact with the bioactive principles of the medicinal plants, influencing their antioxidant capacity. Although at this moment only preliminary data are available, one can ascertain that metals from different sources have a differentiated influence on the antioxidant capacity of the plants. The content of total phenolics in the extracts of Filipendula vulgaris Moench was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). The samples were dried and sieved to <1mm fractions and then randomised and analysed using atomic absorption apectroscopy. The antioxidant activity in the analysed F. vulgaris samples correlated with the flavonoid content. The total flavonoid content varied from 0.368 mg/ml (Extract 6), to 0.660 mg/ml (Extract 3). Therefore, the antioxidant activity can be described as follows: Extract 3>Extract 4≥Extract 5≥Extract 1>Extract 2≥Extract 6. The antioxidant capacity of F. vulgaris extracts was significantly higher in the samples with lower calcium content, i.e. growing in the soils of Area 2, that are poorer in CaCO3 (0.53%) than those from Area 1 (3.13%) and Area 3 (4.35%). Preliminary results show that F. vulgaris is a species with a high polyphenolic content and with a notable antioxidant capacity. The extracts of this plant are used in traditional medicine, but more studies are necessary to establish the entire complex of bioactive principles, in order to obtain certified products. The method ensures sensibility and reproducibility in the measurement of antioxidant action of hydrolytic compounds.

Keywords:

Filipendula vulgaris, plant extracts, natural antioxidants, antioxidant evaluation, metals, DMPD method, TROLOX


Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Food and Health
Pages: 111-116


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