Journal of Food, Agriculture and Environment

Breakable starch granules in a low-amylose buckwheat (Fagopyrum esculentum Moench) mutant

Author(s):

Marija Gregori *, Ivan Kreft

Recieved Date: 2012-01-27, Accepted Date: 2012-05-08

Abstract:

The structure of buckwheat seed endosperm cells was studied by using Scanning Electron Microscopy (SEM). In this study, morphological differences were observed between normal buckwheat cultivar ‘Siva’ endosperm starch granules and those from a Low-Amylose Mutant (LAM), derived from the same cultivar. A normal buckwheat endosperm contains small polygonal starch granules ranging in size from 4 to 8 µm. The LAM endosperm contained mostly spherical starch granules ranging in size from 3 to 6 µm. Pinpricks are visible on the mutant starch granules’ surface. Under physical pressure, while preparing samples for SEM, mutant starch granules are often squashed, but in a similar situation control buckwheat starch granules persist in their original shape. SEM image analysis is a useful tool for classification of individual buckwheat grains in the mixtures of low and normal amylose material and could be used in plant breeding and grain processing to distinguish and separate normal from low-amylose starch endosperm seed material. Spherical native starches with a granule diameter similar to those of lipid micelles have a potential for use as fat replacers with or without further grinding. With the growing demand for carbohydrate-based fat replacers, starch from buckwheat LAM may find commercial applications in food products. 

Keywords:

Buckwheat starch, Fagopyrum esculentum, Low-Amylose Mutant, Scanning Electron Microscopy, starch granule morphology, buckwheat breeding

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Food and Health
Pages: 258-262

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