Journal of Food, Agriculture and Environment

Remaining dynamics of Cry1Ab proteins from transgenic Bt corn in soil

Author(s):

Qingling Fu, Yunhong Zhang, Wei Huang, Hongqing Hu ^*, Diqin Chen, Chang Yang

Recieved Date: 2011-09-22, Accepted Date: 2012-01-06

Abstract:

Genetically modified plants and their residues may impose direct effects on running processes of ecosystem. With the extension of various transgenic Bt crops, their ecological problems have increasingly drawn great attention in academic field recently from all the world, and the focus of this research falls on whether Bt toxin would remain in soil chronically. The remaining amount of Cry1Ab protein from different tissues-roots, leaves and stems-of transgenic Bt corn in yellow cinnamon soil (Alfisol) and red soil (Ultisol) in lab condition was studied by Enzyme-linked Immunosorbent Assay (ELISA) method, and three equations (exponential model, bi-exponential model and shift-log model) were cited to investigate the remaining dynamics. The results showed that the remaining dynamics of Cry1Ab protein from different Bt corn tissues were consistent and analogous, showing two-stage pattern: the stages of rapidly declining and slow-stable. The degradation rate of Cry1Ab protein from the same tissues of Bt corn in two soils had significant difference, with higher remaining protein in yellow cinnamon soil than that in red soil, particularly during the first stage. The remaining amount of Cry1Ab protein from different tissues of Bt corn in the same soil followed the order of roots > leaves > stems during the whole incubation period and was affected by adsorption and incubation time. The bi-exponential model fitted the decomposition of Bt toxin of transgenic Bt corn much better and closely than the exponential model and shift-log model. The DT₅₀ and DT₉₀ calculated from bi-exponential of different tissues varied from 1.2 to 4.9 d and from 18.5 to 46.9 d, respectively. The present data here indicate that the remaining amount of Cry1Ab protein was related to the tissues of the Bt plant and soil properties. These findings will help to evaluate the environmental risk of Bt plant in soil ecosystem and underpin improved planning of management strategies for growing Bt plant.

Keywords:

Transgenic Bt corn, Cry1Ab proteins, remaining dynamics, simulation model

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Agriculture
Pages: 294-298

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