Journal of Food, Agriculture and Environment

Participation of different gamma irradiation techniques, doses and radioprotective compounds upon carbon metabolism, polypeptide upregulation and subsequent 1,3-β-glucans over-production by Pleurotus ostreatus mushrooms

Author(s):

 M. E. A. Dawoud

Recieved Date: 2011-09-04, Accepted Date: 2012-01-10

Abstract:

Commonly cultivated mushrooms of the genus Pleurotus are interesting because of it’s 1,3-β-glucans demonstrating great immunomodulation, antioxidant, anti-inflammatory and analgesic properties. The productivity of these mushrooms depends upon the growing conditions, nutritional substrates, biotic and abiotic stress factors that result in an enzymes over-expression. The present study shows the participation of different techniques and doses of gamma irradiation on carbon metabolism, 1,3 β-glucan synthase enzyme activity and subsequent 1,3 β-glucan over production (both in absence or presence of radioprotective cysteine biocompound). Gradual gamma irradiation (0.5 and 1.0 kGy) significantly enhanced high growth, biomass yield, 1,3 β-glucan production and carbon metabolites contents (carbeome ex. total 1,3 β-glucans, polysaccharides, and carbohydrate metabolism), glucose absorption, 1,3 β-glucan synthase activity and protein content. On the other hand, these low irradiation doses inhibited the accumulation of cellular, extracellular keto acids, free ammonia, extracellular 1,3 β-glucan permeation and vice versa. SDS-PAGE electrophoresis showed that, in absence of radioprotective compound (cysteine), higher gamma irradiation doses (2.0, 2.5, and 3.0 kGy) downregulated original intense protein bands (29.0, 35.0, 47.0 and 53.0 but not 68.0 KDa ) and original light protein bands (80, 88, 92 but not 15.0, 19.0, 40, 55 and 70 KDa). Conversely, addition of 0.1 gm. L^-1 radioprotective compound nullified this effect (except for 29.0 and 47.0 KDa under the effect of 2.5 and 3.0 kGy dose only). Furthermore, different gradual gamma irradiation doses resulted in an intensification of 40 KDa and 15.0 KDa (in both presence and absence of radioprotective compound). 1,3 β-glucan production was increased till 1.5 kGy exposure dose in absence of radioprotective compound but in presence of the radioprotective compound the increase in 1,3 β-glucan was demonstrated under irradiation dose up to 2.0 kGy.

Keywords:

Pleurotus ostreatus,  mushroom, gradual and abrupt gamma irradiation, cysteine radioprotective compounds, carbohydrate metabolism, 1,3 β-glucan synthesis, SDS-PAGE electrophoresis, 1,3 β-glucan synthase

---

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Food and Health
Pages: 235-244

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :