Journal of Food, Agriculture and Environment

Vol 10, Issue 1,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Participation of different gamma irradiation techniques, doses and radioprotective compounds upon carbon metabolism, polypeptide upregulation and subsequent 1,3-β-glucans over-production by Pleurotus ostreatus mushrooms


 M. E. A. Dawoud

Recieved Date: 2011-09-04, Accepted Date: 2012-01-10


Commonly cultivated mushrooms of the genus Pleurotus are interesting because of it’s 1,3-β-glucans demonstrating great immunomodulation, antioxidant, anti-inflammatory and analgesic properties. The productivity of these mushrooms depends upon the growing conditions, nutritional substrates, biotic and abiotic stress factors that result in an enzymes over-expression. The present study shows the participation of different techniques and doses of gamma irradiation on carbon metabolism, 1,3 β-glucan synthase enzyme activity and subsequent 1,3 β-glucan over production (both in absence or presence of radioprotective cysteine biocompound). Gradual gamma irradiation (0.5 and 1.0 kGy) significantly enhanced high growth, biomass yield, 1,3 β-glucan production and carbon metabolites contents (carbeome ex. total 1,3 β-glucans, polysaccharides, and carbohydrate metabolism), glucose absorption, 1,3 β-glucan synthase activity and protein content. On the other hand, these low irradiation doses inhibited the accumulation of cellular, extracellular keto acids, free ammonia, extracellular 1,3 β-glucan permeation and vice versa. SDS-PAGE electrophoresis showed that, in absence of radioprotective compound (cysteine), higher gamma irradiation doses (2.0, 2.5, and 3.0 kGy) downregulated original intense protein bands (29.0, 35.0, 47.0 and 53.0 but not 68.0 KDa ) and original light protein bands (80, 88, 92 but not 15.0, 19.0, 40, 55 and 70 KDa). Conversely, addition of 0.1 gm. L-1 radioprotective compound nullified this effect (except for 29.0 and 47.0 KDa under the effect of 2.5 and 3.0 kGy dose only). Furthermore, different gradual gamma irradiation doses resulted in an intensification of 40 KDa and 15.0 KDa (in both presence and absence of radioprotective compound). 1,3 β-glucan production was increased till 1.5 kGy exposure dose in absence of radioprotective compound but in presence of the radioprotective compound the increase in 1,3 β-glucan was demonstrated under irradiation dose up to 2.0 kGy.


Pleurotus ostreatus,  mushroom, gradual and abrupt gamma irradiation, cysteine radioprotective compounds, carbohydrate metabolism, 1,3 β-glucan synthesis, SDS-PAGE electrophoresis, 1,3 β-glucan synthase

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Food and Health
Pages: 235-244

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