Journal of Food, Agriculture and Environment

Acclimatization related proteins and factors in somaclone lines in kiwifruit (Actinidia deliciosa)

Author(s):

Sayed Sharam Miraghaee ¹, Ali Mostafaie ¹, Danial Kahrizi ^1-3*

Recieved Date: 2011-09-19, Accepted Date: 2012-01-04

Abstract:

Kiwifruit is adapted to Ramsar region (north of Iran) with high relative humidity. Twenty somaclone lines were collected and transplanted from Ramsar to Sahneh region (west of Iran) with moderate and low relative humidity. After five years of transplantation, only one clone acclimatized to new conditions, grown and set fruit and seed successfully. Current study was focused on ecological adaptation related proteins and factors (such as leaf proline, Soluble leaf sugar, Chlorophyll a and b and relative water content) in this line and comparison with stock plant in Ramsar. For this purpose this research was conducted to comparison of electerophoretic protein patterns and main physiological traits in kiwifruit from Ramsar and Sahneh. Results of SDS-PAGE and two-dimensional electrophoresis showed maximal and minimal differences for leaf and seed proteins respectively. Based on results, actinidin, as main protease of kiwifruit, express in fruit of Ramsar line and leaves of Sahneh kiwifruit line mainly. Its expression was relatively low in leaves of Ramsar plant and fruit of Sahneh line. Statistical analysis showed significant differences between kiwifruit of Sahneh and its stock plant in Ramsar for proline, sugar, total protein (of seed, fruit and leaf) and chlorophyll content. Correlation between sugar and proline content was positive and significant (0.931). Total protein of leaf and fruit was correlated with proline significantly and negatively (0.879 and 0.835 respectively). It is concluded that protein expression in kiwifruits was affected by environmental conditions seriously. Switch on and off and down or up regulating of some proteins may be in adaptation process.

Keywords:

Kiwifruit, protein electrophoresis, actinidin, adaptation

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Food and Health
Pages: 198-202

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