Journal of Food, Agriculture and Environment

Sequence analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated in China during 1996 to 2011

Author(s):

Yin Dai ¹, Junjun Wang ², Fazhi Xu ³, Ruihong Zhao ¹, Xiaomiao Hu ¹, Danjun Zhang ^1*

Recieved Date: 2011-10-11, Accepted Date: 2012-01-03

Abstract:

To understand the molecular characteristics of H9N2 avian influenza viruses (AIV), we studied 21 amino acid sequences of haemagglutinin (HA) genes, which were gained from strains in different areas of China during 1996 to 2011. There were 99 variable amino acid residues, accounting for 17.7% of all amino acids. Nine polymorphic amino acid residues (score≥4) were observed in the chicken HA, the HA1 domain showed higher level of mutation than the HA2 domain. Moreover, the result showed that the 3 sites (218-220, 298-300, 305-307) were variable in 7 potential glycosylation positions. There were 7 receptor-binding sites, especially the site 198 with high degree of mutation frequency located in the globular head of protein model. It revealed the site 198 might be more conducive to changing AIV virus to adapt to new hosts and to evade the body’s immune system. Phylogenetic tree showed that no significant regional differentiation was found in HA amino acid sequences of strains. It indicated that geographical isolation of virus could be broken by extensive trades and migration of birds.

Keywords:

Avian influenza virus, H9N2 subtype, hemagglutinin gene, sequence analysis

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Food and Health
Pages: 143-146

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